Conference Day 1
Wednesday, May 10 2023
8:00 am
Welcome Coffee and Registration
9:00 am
Chair’s Opening Remarks
Optimizing Cell Line Development to Increase Scalability
9:15 am Fireside Chat: Discussing the Hurdles in Developing a Fully Scalable Cell Line
Synopsis
- Exploring approaches towards increasing the scalability of established cell lines
- Tackling density thresholds, cell media and productivity decreases
- Regaining control of cell lines, reducing genetic drift, and increasing stability
10:00 am Enhancing Cell Line Development & Bioprocess Optimization with Actionable Omics, AI, & Systems Biology
Synopsis
- Understanding that high-throughput technologies are inherently observational, but can be adapted to inform cell line development and process optimization
- Achieving actionable omics through properly designed experiments, AI, and systems biology to increase productivity
- Integrating omics with an understanding of the cellular machinery, allowing for a connection between the intracellular metabolism and bioreactors or media to increase bioprocessing efficiency
10:30 am
Morning Refreshments & Speed Networking
Innovation in Cell Line Engineering to Regain Control of Expression
11:30 am Highlighting the Power of Using Transposases for Targeted Gene Integration
Synopsis
- Encapsulating the ability of engineered transposases as a newer, faster and safer gene integration tool
- Understanding the biological implications and mechanisms of semi site-specific integration
- Exploring the impact of using transposases for gene editing on the timeline and scalability of Advanced Therapy Medicinal Products
12:00 pm Achieving Extremely Efficient Transgene Insertion to Gain Control of Expression
Synopsis
- Using Sleek technology to increase the efficiency of gene knock-in via CRISPR
- Harnessing the security and promoter strength of essential genes to gain control over expression
- Reducing off target effects and variable yields with precise gene insertion
12:30 pm Extracting the Capacity of Automated Sense & Response Mechanisms to Control Conditional Gene Expression
Synopsis
- Exploring how automated sense and response mechanisms can be used to optimise productivity and increase reliability of expression platforms
- Understanding how aiming for inducible expression in mammalian cell lines could achieve greater control over expression levels and increase the versatility of various cell lines
- Reducing toxicity and increasing productivity to enhance better scale up
1:00 pm
Lunch & Networking
Mitigating Cell Line Development Risks to Accelerate Regulatory Approval
2:30 pm Navigating the Pathway to Regulatory Approval for Advanced Therapies Using Cell Line Development
Synopsis
- Laying out the analytical measures and regulatory considerations required to produce a high quality product using mammalian cell lines
- Exploring the changes in approach and analysis needed when using nonmammalian cell lines
- Elucidating how variations in plasmid sources and choice of vector affect the regulatory hurdles encountered. Covering LVs, AAV and Anelloviral therapies
3:00 pm Assessing the Usefulness and Quality of the Stable Pool Approach for Achieving Clinic
Synopsis
- Contrasting the stable cell pool approach with traditional methods in terms of scalability and speed
- Using random integration to achieve biophysical and biochemical comparability to clones produced via target integration
- Demonstrating how screening, assays and stability studies can be employed to reduce the risks posed
3:30 pm
Afternoon Poster Session & Networking
Understanding the Biological Hurdles to Increase Productivity
4:30 pm Panel: Tackling the Biological Hurdles Presented at Scale Up
Synopsis
- Addressing how the low yield caused by cell density and aggregation can be overcome
- Eliminating batch variability: Why scale out is less preferable and less consistent
- Regaining oxygen control at industrial scale to enhance cell growth and productivity
5:15 pm Employing Virus Based Delivery Platforms to Fine Tune the Expression Casette
Synopsis
- Isolating limiting components to enhance the multi-component genetic circuit for unnatural amino acid incorporation.
- Adapting virus assisted directed evolution of engineered tRNAs in mammalian cells to increase scalability
- Progressing development of stable cell lines for unnatural amino acid incorporation